Primocin® is designed to offer complete protection of primary cells from microbial contamination. Primocin® is a broad-spectrum antibiotic formulation that is gentle on your cells but lethal to the microbes. There are a number of common sources of microbial contamination especially in the isolation of primary cells from both animal and human tissue, including commensal flora and/or subclinical infections.

Key Features:

• Active against bacteria, mycoplasma, and fungi
• A common addition to various standardized primary cell culture protocols
• Used throughout culturing process (e.g. culture media, wash solutions, and  biopsy storage media) 
• Highly cited in the literature

rimocin® is composed of four compounds, of which three, act directly on Gram-positive bacteria, Gram-negative bacteria, and mycoplasmas. The primary mode of action for these compounds is to block microbial DNA and protein synthesis. The fourth compound in Primocin® eradicates fungi, including yeasts, by directly targeting processes at the fungal membrane.

Primocin® is successfully used in many types of primary cell cultures (see citations) including:

• Differentiated mouse- and human-derived cells such as astrocytes and immune cells (e.g. natural killer cells)
• Stem cells such as mouse- and human-derived embryonic and pluripotent stem cells
• 3D cellular models (i.e. organoid and spheroids) such as colon epithelial and carcinoma organoids

Primocin® is made in-house at InvivoGen and is of the highest quality with rigorous quality control performed to ensure the absence of lot-to-lot variation.

Specifications

Product concentration: 50 mg/ml

Recommended working concentration: 100 μg/ml

• 1 ml vial of Primocin® is sufficient for 500 ml of culture.
• 20 ml bottle of Primocin® is sufficient for 10 liters of culture.

Quality Control:

• Physicochemical characterization (pH and appearance) has been confirmed
• Endotoxin levels: < 0.5 EU/mg
• Potency has been validated on bacterial and fungal reference strains

Contents

Primocin® is provided as a cell culture tested, sterile filtered, light yellow solution at a concentration of 50 mg/ml.

This product is available in three pack sizes:

• ant-pm-05 - 5 x 1 ml vial (250 mg) 
• ant-pm-1 - 10 x 1 ml vial (500 mg)
• ant-pm-2 - 1 x 20 ml bottle (1 g)

Primocin® is shipped at room temperature.
Upon receipt Primocin® can be stored at 4°C for 3 months or at -20°C for long term storage

Details

Use of Primocin® in primary cell cultures

Primocin® is frequently cited in the literature for use in the protection of a number of different primary cell cultures. This includes (but is not limited to):

• Numerous human and murine primary cell cultures, including fibroblasts [1], glial cells [2], astrocytes [3], and immune cells (e.g. NK cells) [4].
• Culturing and reprogramming of human and murine embryonic cells [5] and pluripotent stem cells [6].
• 3D cell cultures including colon epithelial and carcinoma organoids [7] as well as breast, bladder, and prostate cancer organoids [8-10].
• A number of published standardized protocols such as tumor organoid-T-cell co-culture systems [11].