PRKG1 Polyclonal Antibody | E-AB-60735

PRKG1 Polyclonal Antibody | E-AB-60735 | Gentaur UK, US & Europe Distribution

Type: Polyclonal Antibody

Synonyms: PRKG1, AAT8, PKG, PRKG1B, PRKGR1B, cGK, cGK 1, cGK1, cGKI, cGKI-BETA, cGKI-alpha

Application: WB, IHC

Reactivity: Human, Mouse

Host: Rabbit

Isotype: IgG

Reserch Areas: Cancer, Cardiovascular, Signal Transduction

Background: Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Recombinant fusion protein of human PRKG1 (NP_006249.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000 IHC 1:50-1:200

Clone: N/A

Conjugation: Unconjugated

Molecular Weight(Calculated): 44 kDa/76 kDa/77 kDa

Molecular Weight(Observed): 65 kDa