PRKR Antibody| F40175-0.4ML

PRKR Antibody| F40175-0.4ML | Gentaur UK, US & Europe Distribution

Background: Interferon-induced, double-stranded RNA-activated protein kinase is a serine-threonine kinase. Activation by dsRNAs leads to autophosphorylation of PRKR and allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), leading to the inhibition of protein synthesis. Human gamma-interferon (IFNG) mRNA exploits localized activation of PRKR in the cell to regulate its own translation. IFNG mRNA activates PRKR through a pseudoknot in its 5-prime untranslated region. The HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PRKR and the translation initiation factor EIF2-alpha, a target of PRKR. E2 inhibits the kinase activity of PRKR and blocks its inhibitory effect on protein synthesis and cell growth, which provides one mechanism by which HCV may circumvent the antiviral effect of interferon.

Format: Purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human, Mouse

Predicted Spacies Reactivity: N/A

Application: WB, IHC, ELISA

Application Details: Western blot: 1:1000, IHC (Paraffin): 1:50-1:100

Application Note: The stated application concentrations are suggested starting amounts. Titration of the PRKR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This PRKR antibody is available for research use only.

Purity: Purified

Immunogen: A portion of amino acids 519-550 from the human protein was used as the immunogen for this PRKR antibody.

Storage: Aliquot the PRKR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A