Pro-MMP-2, mouse | 7780

The proGelatinase A, a member of the matrix metalloproteinase (MMP) family, has been isolated from macrophages and fibroblasts. Gelatinase A hydrolyses several components of the extracellular matrix, e.g. the collagen types IV, V and XI and Gelatin. ProGelatinase A complexed via their C-terminal domain with TIMP-2 was isolated from culture media of different cell types. This complex shows both properties of its constituents: Like TIMP-2 it inhibits active matrix metalloproteinases and like Gelatinase it shows proteolytic activity after activation with APMA (4-aminophenylmercury acetate). However, its proteolytic activity is less than 10% of that of Gelatinase A not complexed with TIMP-2. In contrast to the other MMPs the proGelatinase A cannot be activated by the serine proteinase trypsin. Until quite recently a potential natural activator that can transform latent proGelatinase A into the active form was unknown. It was shown that the catalytic domain of the membrane type 2-matrix metalloproteinase activates proGelatinase A as well as the proGelatinase A / TIMP-2 complex, by cleaving the 72 kDa proGelatinase A to yield 67 kDa Gelatinase A, which is than transformed into 62 kDa Gelatinase A. The 62 kDa form is about twice as active as the 67 kDa form towards the Dnp-pepitde (Masui et al.). No significant difference in activity was found between free and complexed Gelatinase A forms.

7780 | Pro-MMP-2 mouse DataSheet

Biomolecule/Target: N/A

Synonyms: Matrix metalloproteinase-2, MMP, MMP recombinant, MMP protein, MMP inhibitor

Alternates names: 72 kDa type IV collagenase (EC 3.4.24.24) (72 kDa Gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) [Cleaved into: PEX]

Taglines: A Protein involved in breakdown of extracellular matrix

NCBI Gene ID #: 8600

NCBI Gene Symbol: OPGL

Gene Source: Human

Accession #: O14788

Recombinant: No

Source: mouse fibroblasts

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent stimulation of IL-8 production by human PBMC is <10 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 40/42 kDa

Concentration: 50 g/ ml; >40 mU/mg (Masui substrate), >40 mU/mg (Knight substrate) - APMA activation, 1 hour/37oC

Appearance: Liquid

Physical form description: In 50 mM Tris-HCl, pH 7; 200 mM NaCl; 5 mM CaCl; 1 M ZnCl; 0.05% Brij 35; 0,05% NaN

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg /ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A