26
Procathepsin B, Human Recombinant | 7579
- SKU:
- 26-7579-GEN
- Availability:
- Usually shipped in 5 working days
Description
Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer. s Procathepsin B can be auto-catalytically activated to mature Cathepsin B (~28 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at room temperature (RT). The enzyme is fully activated within 3-4 h of incubation at RT. After activation mature Cathepsin B is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.
7579 | Procathepsin B Human Recombinant DataSheet
Biomolecule/Target: Procathepsin B
Synonyms: CTSB, CPSB
Alternates names: Angiopoietin-1
Taglines: A lysosomal protease playing a role in protein degradation, arthritis, apoptosis, and cancer.
NCBI Gene ID #: 284
NCBI Gene Symbol: ANGPT1
Gene Source: Human
Accession #: Q15389
Recombinant: Yes
Source: HeLa cells
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC).
Biological activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC).
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 60-70 kDa
Concentration: N/A
Appearance: Lyophilized
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Phosphate, pH 7.5, 200 mM NaCl, 5% Trehalose.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: SNQRRSPENS GRRYNRIQHG QCAYTFILPE HDGNCRESTT DQYNTNALQR DAPHVEPDFS SQKLQHLEHV MENYTQWLQK LENYIVENMK SEMAQIQQNA VQNHTATMLE IGTSLLSQTA EQTRKLTDVE TQVLNQTSRL EIQLLENSLS TYKLEKQLLQ QTNEILKIHE KNSLLEHKIL EMEGKHKEEL DTLKEEKENL QGLVTRQTYI IQELEKQLNR ATTNNSVLQK QQLELMDTVH NLVNLCTKEG VLLKGGKREE EKPFRDCADV YQAGFNKSGI YTIYINNMPE PKKVFCNMDV NGGGWTVIQH REDGSLDFQR GWKEYKMGFG NPSGEYWLGN EFIFAITSQR QYMLRIELMD WEGNRAYSQY DRFHIGNEKQ NYRLYLKGHT GTAGKQSSLI LHGADFSTKD ADNDNCMCKC ALMLTGGWWF DACGPSNLNG MFYTAGQNHG KLNGIKWHYF KGPSYSLRST TMMIRPLDFH HHHHH