Procathepsin K, human recombinant | 1026

Cathepsin K, a member of the papain cysteine proteinase family is the predominant proteinase responsible for the resorption of the bone matrix. Cathepsin cleaves proteins such as collagen type I, collagen type II and osteonectin, thereby playing a role in bone remodeling and resorption in osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is synthesized as an inactive proenzyme (35.1 kDa) that is converted to its mature active form (23.6 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic.

1026 | Procathepsin K, human recombinant DataSheet

Biomolecule/Target :Procathepsin K

Synonyms: CTSK, CTSO, CTSO3

Alternates names: CTSK, CTSO, CTSO3

Taglines: Predominant proteinase responsible for the resorption of the bone matrix.

NCBI Gene ID #: 1513

NCBI Gene Symbol: CATK

Gene Source: Human

Accession #: P43235

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGE: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The specific activity is >1000 mU/mg (1U = 1 umole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog #K141-100)

Results: The specific activity is >1000 mU/mg (1U = 1 umole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog #K141-100)

Binding Capacity: N/A

Unit Definition: 1U = 1 umole/min/mg

Molecular Weight: 35.9 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0).

Reconstitution Instructions: N/A

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans