Profilin 1, Human Recombinant | P1365

Profilin1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein significantly enhances skin wound healing in-vitro and in-vivo that may be mediated by purinergic receptors. It is also active in endothelial cell migration and vessel sprouting. It is thought to regulate actin polymerization in response to extracellular signals. Recombinant Profilin1 protein was expressed in E.coli and purified by using conventional chromatography techniques

P1365 | Profilin 1 Human Recombinant DataSheet

Biomolecule/Target: Profilin 1

Synonyms: Epididymis tissue protein Li 184a, PFN1

Alternates names: A Proliferating-inducing Ligand, TNFSF13, TRDL-1

Taglines: Ubiquitous actin monomer-binding protein

NCBI Gene ID #: 69583

NCBI Gene Symbol: APRIL

Gene Source: Mouse

Accession #: Q9D777

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to induce cell proliferation of activated T-cells.

Biological activity: Determined by its ability to induce cell proliferation of activated T-cells.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 21.9 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 5 and 100 mM Arginine.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MRREVSRLQR SGGPSQKQGE RPWQSLWEQS PDVLEAWKDG AKSRRRRAVL TQKHKKKHSV LHLVPVNITS KDSDVTEVMW QPVLRRGRGL EAQGDIVRVW DTGIYLLYSQ VLFHDVTFTM GQVVSREGQG RRETLFRCIR SMPSDPDRAY NSCYSAGVFH LHQGDIITVK IPRANAKLSL SPHGTFLGFV KL