Progesterone ELISA Kit - K7416

K7416 | Progesterone ELISA Kit DataSheet

’s Progesterone ELISA kit is a competitive immunoassay for the quantitative determination of Progesterone in biological fluids. The kit uses a polyclonal antibody to Progesterone to bind, in a competitive manner, Progesterone in a sample or Progesterone which has an alkaline phosphatase molecule covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405 nm. The intensity of the bound yellow color is inversely proportional to the concentration of Progesterone in either standards or samples. The measured optical density for the samples is used to calculate the concentration of Progesterone in the sample. Sensitivity: 8.57 pg/ml (range: 15.62-500 pg/ml). The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Cross Reactivity: Progesterone:100%, 5-Pregnane-3,20-dione: 100%, 17-OH-Progesterone: 3.46%, 5 alpha-Pregnen-3ß-o1-20-one: 1.43%, Corticosterone: 0.77%, 4-Androstene-3,17-dione: 0.28%, Deoxycorticosterone: 0.056%, DHEA: 0.013%, 17ß-Estradiol: <0.001%, Estrone: 0.001%, Estriol: <0.001%, Testosterone: <0.001%, Hydrocortisone: <0.001%, 5 alpha-Pregnane-3,20-diol: <0.001%, Danazol: <0.001%.

Alternate Name : P4 ELISA Kit

Tag Line : Sensitive, Colorimetric Assay

Summary : • Detection method- Absorbance (405 nm) • Species reactivity- all • Application- Quantitative detection of progesterone

Detection Method : Absorbance (405 nm)

Sample Type : Serum, saliva, tissue culture media

Species Reactivity : all

Applications : This ELISA kit is used for quantitative detection of progesterone

Features and Benefits : • Easy, convenient and time-saving method to measure Progesterone from all species • Sensitivity: 8.57 pg/ml (range: 15.62-500 pg/ml)