Prolactin, rat recombinant | 4689

Prolactin (also known as mammotrophin, luterotropic hormone, and lutetropin) is a neuroendocrine hormone secreted by the pituitary gland. Its primary function is to promote and maintain lactation during pregnancy and suckling. In addition, Prolactin plays an immune-regulatory role by stimulating the activities of ornithine decarboxylase and protein kinase C, which are important for the proliferation, differentiation, and function of lymphocytes. Recombinant murine Prolactin is a 22.5 kDa globular protein containing 198 amino acid residues.

4689 | Prolactin rat recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Mammotropin, Luterotropic hormone, Lutetropin, Prlr, PRL, luteotropic hormone, LTH, PRLR, PRL-R, PRL R

Alternates names: CT-1

Taglines: An important regulator of immune system with cell cycle related functions

NCBI Gene ID #: 13019

NCBI Gene Symbol: CT1

Gene Source: Mouse

Accession #: Q60753

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.

Biological activity: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 21.3 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.5 mg/ml in 5 mM Tris, pH 8.0

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: SQREGSLEDH QTDSSISFLP HLEAKIRQTH NLARLLTKYA EQLLEEYVQQ QGEPFGLPGF SPPRLPLAGL SGPAPSHAGL PVSERLRQDA AALSVLPALL DAVRRRQAEL NPRAPRLLRS LEDAARQVRA LGAAVETVLA ALGAAARGPG PEPVTVATLF TANSTAGIFS AKVLGFHVCG LYGEWVSRTE GDLGQLVPGG VA