Prolactin Receptor, rat recombinant | 4991

Prolactin is a pituitary hormone that plays a role in the stimulation of milk production, salt and HO regulation, growth, development and reproduction. The primary step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. Prolactin is a hormone involved in a range of significant functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. Prolactin exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane Prolactin receptor. PRLR varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. Recombinant human Prolactin Receptor (Extra Cellular Domain) produced in E.Coli is a non-glycosylated, Polypeptide chain containing 206 amino acids and having a molecular mass of 24.12 kDa.

4991 | Prolactin Receptor rat recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: PRL-R, Prolactin receptor, Lactogen receptor, Prlr, PRL, luteotropic hormone, LTH, PRLR, PRL-R, PRL R

Alternates names: BTC

Taglines: A cytokine receptor

NCBI Gene ID #: 12223

NCBI Gene Symbol: BTC

Gene Source: Mouse

Accession #: Q05928

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of mouse Balb/3T3 cells. The expected ED50 is 0.01 ng/ml, corresponding to a specific activity of 1 x 108 units/mg.

Biological activity: Determined by its ability to stimulate the proliferation of mouse Balb/3T3 cells. The expected ED50 is 0.01 ng/ml, corresponding to a specific activity of 1 x 10^8 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 9 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 °C to -80 °C.

Amino acid sequence: DGNTTRTPET NGSLCGAPGE NCTGTTPRQK VKTHFSRCPK QYKHYCIHGR CRFVVDEQTP SCICEKGYFG ARCERVDLFY