Prostaglandin E1 (PGE1) ELISA Kit - E4716

E4716 | Prostaglandin E1 (PGE1) ELISA Kit DataSheet

's PGE1 ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE1. During the reaction, PGE1 in the sample or standard competes with a fixed amount of PGE1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGE1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase(HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternate Name :

Tag Line : The competitive ELISA Kit for in vitro quantitative determination of PGE1 concentrations in serum, plasma and other biological fluids.

Summary :

Detection Method : Absorbance-450 nm

Sample Type : Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids, Tissue and cell lysates

Species Reactivity : All

Applications : Specificity: This kit recognizes PGE1 in samples. No significant cross-reactivity or interference between PGE1 and analogues was observed., Detection Range: 78.13—5000 pg/mL, Repeatability: Coefficient of variation is < 10%., in vitro quantitative determination of PGE1 concentrations in serum, plasma and other biological fluids., Sensitivity: 46.88 pg/mL

Features and Benefits :