Molecular purification methods such as immunoprecipitation (IP), co-IP and chromatin IP are important tools in studying lowly expressed proteins, protein-protein interactions or protein-nucleic acid interactions. Santa Cruz Biotechnology offers a comprehensive array of research support products for purification techniques. Protein A/G PLUS is an essential specialty reagent used for isolating immunoglobulins from nearly any mammalian species. Protein A/G PLUS is a genetically-engineered protein that combines binding profiles of Protein A and Protein G, thereby producing an exceptionally high-capacity reagent. Protein A/G PLUS works by covalently linking to the Fc region of mammalian antibody isotypes, specifically IgG, IgA, IgE and IgM. Protein A/G PLUS is a powerful reagent for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from other serum proteins. Protein A/G PLUS is linked to a high-quality Agarose resin, CL-6B, which makes it a superior and premium product that produces high purity and purification yield of IgGs from mammalian samples.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

• Provides binding for all species of antibody and IgG subclasses recognized by either Protein A or Protein G
• provided as 0.5 ml agarose/2.0 ml; 100 reactions
• suitable for use at 20 µl per immunoprecipitation reaction
• binding capacity is 10 mg of antibody per 1 ml of packed beads, settled not spun
• pre-treated to reduce non-specific immunoglobulin binding
• linked to Sepharose CL-6B
• strong binding to mouse IgG₁ , mouse IgG_2a , IgG_2b and IgG₃ , rat IgG_2a and IgG_2c , human IgG₁ , IgG₂ , IgG₃ and IgG₄ , rabbit, goat, bovine, porcine, ovine, canine, feline and equine IgG
• weak to medium binding to rat IgG₁ , rat IgG_2b , human IgM, IgA and IgE
• pre-washed and ready to use