Description
Protein A Magnetic Beads are prepared by covalently coupling Recombinant Protein A (contains five IgG binding domain, BV catalog # 6500B) to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity for IgG. The capacity of IgG binding is generally greater than 25 mg of human IgG per ml of wet gel.
6507 | Protein A Magnetic Beads DataSheet
Sort Name: Protein A Magnetic Beads
Label Name: N/A
Taglines: Reusable Protein A conjugated magnetic beads with ≥25 mg/ml binding capacity for antibody purification from multiple sources
Product Highlights: •CONTENTS- Supplied as a 50% slurry in PBS with 0.02% sodium azide. •TECHNICAL SPECIFICATIONS- Parameter Description: Support Characteristics- Paramagnetic, spherical, 6 % cross-linked agarose Ligand- Recombinant Protein A Particle Size- 75 – 150 µm Binding Capacity- Generally >25 mg human IgG/ml wet beads Working Temperature-Room temperature Storage Solution- PBS w/0.02% NaN₃ Storage Temperature-4 – 8 °C •FEATURES- Easy to use, high-binding capacity, non-adherent beads. Useful for immunoprecipitation and enrichment of IgG antibodies. High affinity for Fc region of IgG antibodies from a variety of species. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
Packaging: EA
Additional Information
Storage Temperature: |
+4°C |
Shipping: |
Gel Pack |
Shelf Life: |
12 months |