Protein Disulfide Isomerase (PDI), human recombinant | 7601

Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. Recombinant Human PDI produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 508 amino acids and having a molecular mass of 57.1 kDa. The human PDI contains N-terminal 6xHis-tag and is purified by proprietary chromatographic techniques.

7601 | Protein Disulfide Isomerase PDI human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Protein Disulfide Isomerase

Alternates names: Semaphorin-4D, BB18, A8, GR3

Taglines: Catalyzes disulfide exchange and protein folding

NCBI Gene ID #: 10507

NCBI Gene Symbol: CD100

Gene Source: Human

Accession #: Q92854

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Measured by its ability to inhibit chemokine (hMCP-3) induced human monocyte migration.

Biological activity: Measured by its ability to inhibit chemokine (hMCP-3) induced human monocyte migration.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 78.9 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: FAPIPRITWE HREVHLVQFH EPDIYNYSAL LLSEDKDTLY IGAREAVFAV NALNISEKQH EVYWKVSEDK KAKCAEKGKS KQTECLNYIR VLQPLSATSL YVCGTNAFQP ACDHLNLTSF KFLGKNEDGK GRCPFDPAHS YTSVMVDGel YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIRKSPDS PDGEDDRVYF FFTEVSVEYE FVFRVLIPRI ARVCKGDQGG LRTLQKKWTS FLKARLICSR PDSGLVFNVL RDVFVLRSPG LKVPVFYALF TPQLNNVGLS AVCAYNLSTA EEVFSHGKYM QSTTVEQSHT KWVRYNGPVP KPRPGACIDS EARAANYTSS LNLPDKTLQF VKDHPLMDDS VTPIDNRPRL IKKDVNYTQI VVDRTQALDG TVYDVMFVST DRGALHKAIS LEHAVHIIEE TQLFQDFEPV QTLLLSSKKG NRFVYAGSNS GVVQAPLAFC GKHGTCEDCV LARDPYCAWS PPTATCVALH QTESPSRGLI QEMSGDASVC PDKSKGSYRQ HFFKHGGTAE LKCSQKSNLA RVFWKFQNGV LKAESPKYGL MGRKNLLIFN LSEGDSGVYQ CLSEERVKNK TVFQVVAKHV LEVKVVPKPV VAPTLSVVQT EGSRIATKVL VASTQGSSPP TPAVQATSSG AITLPPKPAP TGTSCEPKIV INTVPQLHSE KTMYLKSSDN R