Proteinase K, Recombinant, 20 mg/ml Solution, Molecular Grade | 9211

A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).

9211 | Proteinase K Recombinant 20 mg/ml Solution Molecular Grade DataSheet

Biomolecule/Target: N/A

Synonyms: Endopeptidase K, Tritirachium alkaline proteinase

Alternates names: Cutaneous T-cell Attracting Chemokine, CCL27, ALP, Skinkine, Eskine

Taglines: A serine protease which inactivates nucleases

NCBI Gene ID #: 20301

NCBI Gene Symbol: Ccl27

Gene Source: Murine

Accession #: Q9Z1X0

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to chemoattract human peripheral blood lymphocytes using a concentration range of 10.0-100.0 ng/ml.

Biological activity: Determined by its ability to chemoattract human peripheral blood lymphocytes using a concentration range of 10.0-100.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 10.9 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: LPLPSSTSCC TQLYRQPLPS RLLRRIVHME LQEADGDCHL QAVVLHLARR SVCVHPQNRS LARWLERQGK RLQGTVPSLN LVLQKKMYSN PQQQN