Proteinase K, Recombinant, Standard Grade | 9250

A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF). It is not inhibited by iodoacetic acid, the trypsin-specific inhibitor TLCK, the chymotrypsin-specific inhibitor TPCK, and p-chloromercuribenzoate.

9250 | Proteinase K Recombinant Standard Grade DataSheet

Biomolecule/Target: N/A

Synonyms: Protease K, Endopeptidase K, Standard Grade Proteinase K, Proteinase K, Tritirachium alkaline proteinase

Alternates names: Connective Tissue Growth Factor-Like protein, CCN5, CT-58

Taglines: Proteinase K, Standard Grade

NCBI Gene ID #: 8839

NCBI Gene Symbol: CTGFL

Gene Source: Human

Accession #: O76076

Recombinant: True

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 is between 10-20 ng/ml in the presence of 15 ng/ml of human IGF-II.

Biological activity: Determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 is between 10-20 ng/ml in the presence of 15 ng/ml of human IGF-II.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 24.3 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not raise to neutral pH. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF