Proteinase K, Recombinant, Standard Grade | 9251

A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF). It is not inhibited by iodoacetic acid, the trypsin-specific inhibitor TLCK, the chymotrypsin-specific inhibitor TPCK, and p-chloromercuribenzoate.

9251 | Proteinase K, Recombinant, Standard Grade DataSheet

Biomolecule/Target :

Synonyms: Proteinase K, Endopeptidase K, Tritirachium alkaline proteinase, Protease K, Standard Grade Proteinase K

Alternates names: Proteinase K, Endopeptidase K, Tritirachium alkaline proteinase, Protease K, Standard Grade Proteinase K

Taglines: Proteinase K, Standard Grade

NCBI Gene ID #: 5621

NCBI Gene Symbol: PROK

Gene Source: Recombinant Pichia pastoris

Accession #: P06873

Recombinant: True

Source: Recombinant Pichia pastoris

Purity by SDS-PAGE: >90%

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method):

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 umole (181ug) of tyrosine per minute at pH 7.5 at 37℃.

Molecular Weight: 28.9

Concentration:

Appearance: Liquid

Physical form description: Liquid

Reconstitution Instructions: Soluble in water ((1–2 mg/ml)

Amino acid sequence:

Handling: Centrifuge the vial prior to opening.

Usage: Molecular biology applications to digest unwanted proteins, such as nucleases from DNA or RNA preparations from microorganisms, cultured cells, and plants.