Protocadherin X Antibody | 42-252

Protocadherin X Antibody | 42-252 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: N/A

Homology: Expected Species Reactivity based on sequence homology: Human

Immunogen: The immunogen for this antibody is: C-KYNWVTTPTTFK

Research Area: Signal Transduction, Neuroscience

Tested Application: E

Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 75kDa band in Human Brain (Amygdala, Hippocampus, Substantia nigra) lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115kDa according to NP_116753.1 and of 113kDa according to NP_055337.1, the smallest reported isoforms. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Specificiy: This antibody is expected to recognize all reported Y-linked isoforms (NP_116753.1; NP_116754.1; NP_116755.1) and all reported X-linked isoforms (NP_055337.1; NP_055337.1; NP_116750.1; NP_116751.1) .

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: PCDH11Y, protocadherin 11 Y-linked, PCDH-PC, PCDH11X, PCDH22, PCDHY, OTTHUMP00000033077, protocadherin 11 X-linked, protocadherin 22, protocadherin Y, RP13-138P15.1, PCDH-X, PCDH11, PCDHX, protocadherin 11X, protocadherin X, protocadherin-S

User Note: N/A

BACKGROUND: N/A