PRR5L Antibody | 6293

PRR5L Antibody | 6293 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Rat: (88%)

Immunogen: PRR5L antibody was raised against a 17 amino acid synthetic peptide near the center of human PRR5L.
The immunogen is located within amino acids 220 - 270 of PRR5L.

Research Area: Apoptosis

Tested Application: E, WB, ICC, IF

Application: PRR5L antibody can be used for detection of PRR5L by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: At least three isoforms of PRR5L are known to exist; this antibody will recognize only isoforms a and b. PRR5L antibody will not cross-react with PPR5.

Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Positive Control 2: Cat. No. 17-010 - HEK293 Cell Slide

Positive Control 3: Cat. No. 17-201 - 3T3/BALB Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: PRR5L Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PRR5L Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PRR5L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: PRR5L Antibody: PROTOR2, PROTOR2, Proline-rich protein 5-like, Protein observed with Rictor-2, Protor-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PRR5L Antibody: Proline-rich protein 5-like (PRR5L) , also known as Protor-2, is structurally similar to PRR5 and also binds to the mTOR complex 2 (mTORC2) . PRR5L is not required for mTORC2 integrity or kinase activity, but dissociates from mTORC2 upon knockdown of tuberous sclerosis complex 1 (TSC1) and TSC2. Knockdown of PRR5L reduced apoptosis in mTORC2 deficient cells as well as cells where mTOR was active, suggesting that mTORC2-dissociated PRR5L may promote apoptosis, and thus play a role in the balance between cell growth and cell death.