PsaC | PSI-C core subunit of photosystem I | AS10 939

PsaC | PSI-C core subunit of photosystem I | AS10 939 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide conserved in all known PsaC proteins including Arabidopsis thaliana Uniprot:P62090 TAIR: AtCg01060, Hordeum vulgare UniProt: P69416, Oryza sativa UniProt: P0C360, Chlamydomonas reinhardtii UniProt: Q00914, Synechococcus elongatus UniProt: Q31QV2

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS04 042P | PsaC | PSI-C core subunit of photosystem I (PsaC antibody + PsaC protein positive control) AS04 042S | PsaC protein standard for quantitation or positive control collection of antibodies to PSI proteinsAS05 084 | Anti-PsbA | D1 protein of PSII, C-terminal (rabbit antibody) (thylakoid membrane marker), rabbit antibodiesPlant and algal protein extraction buffer

Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 9 kDa

Confirmed Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Dactylis glomeRata, Emiliania huxleyi, Euglena gracilis, Gonyaulax polyedra, Horderum vulgare, Mesembryanthemum sp., Spinacia oleracea, Flaveria sp., Heterosigma akashiwo, Micromonas pusilla, Phaeodactylum tricornutum, Porphyra sp., symbiotic dinoflagellates of Stylophora pistillata and Turbinaria reniformis; Synechococcus sp.PCC7002, sp. PCC 7942, Synechocystis sp. PCC 6803, Thalassiosira pseudonana, Thalassiosira punctigera, Trichodesmium erythraeum, Triticum aestivum

Predicted Reactivity: Algae, Cannabis sativa, Chromera velia, Cyanobacteria, Brassica napus, Glycine max, Manihot esculenta, Nannochloropsis sp., Nicotiana benthamiana, Nicotiana tabacum, Physcomitrium patens, Prochlorococcus sp. (surface and a deep water ecotype), Spinacia oleracea, Synechococcus PCC 8801, Thermosynechococcus elongatus (BP-1) Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot.For the most optimal results use:thylakoid membranes or PSI particles, solubilized in a SDS sample buffer (final concentrations: 63 mM Tris HCl, 10% glycerol, 2% SDS, 0.0025% bromophenol blue) with 2.5% beta-mercaptoethanol at 85C for 2 minutes. The samples were spun softly, then the supernatant loaded. This product can be sold containing ProClin if requested.

Background: PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe-S clusters FA and FB through two cysteine-rich domains.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: ATCG01060

Category: Global Antibodies

Research Area: Plant Biology, Photosynthesis