PUMA/bbc3 Antibody | 3339R

PUMA (p53 upregulated modulator of apoptosis) is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA-? and PUMA-?. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

3339R | PUMA/bbc3 Antibody DataSheet

Antibody Target: PUMA

Target Alternative Name: PUMA/JFY1 ,JFY-1, PUMA, BBC3, bcl-2 binding component 3

Tag Line:

Category: PUMA

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human, mouse, rat

Immunogen Sequence: Synthetic peptide surrounding amino acid 177 of human PUMA

Accession #: Q9BXH1

Gene ID: 27113

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-PUMA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Application: Western blot, Immunohistochemistry

Positive Control: Western Blot: Jurkat cells and Rat kidney tissue, IHC: Tonsil tissue

Application And Usages: Western blotting (0.5-4 µg/ml)and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~25 kDa (193 a.a.) PUMA alpha and ~16 kDa (131 a.a.) PUMA beta in samples from human, mouse and rat origins.

Country of Animal Origin: China

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified:

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified