Additional Information
Size:  | 
                                                    100 µg  | 
                                                
Target:  | 
                                                    PUMA  | 
                                                
Conjugate:  | 
                                                    APC  | 
                                                
Research Area:  | 
                                                    Cancer | Apoptosis | Intracellular | Bcl2 Family | Cancer | Invasion/microenvironment | Cell Signaling | Metabolism | Mitochondrial Metabolism  | 
                                                
Alternative Name:  | 
                                                    BBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody  | 
                                                
Category:  | 
                                                    Antibodies  | 
                                                
Product Type:  | 
                                                    Polyclonal Antibody  | 
                                                
Immunogen:  | 
                                                    N-terminal amino acids of human PUMA  | 
                                                
Immunogen Species:  | 
                                                    Human  | 
                                                
Applications:  | 
                                                    WB, IHC  | 
                                                
Host:  | 
                                                    Rabbit  | 
                                                
Isotype:  | 
                                                    N/A  | 
                                                
Species Reactivity:  | 
                                                    Human, Rat, Rainbow Trout (Oncorhynchus mykiss)  | 
                                                
Antibody Dilution:  | 
                                                    WB (1:500), IHC (1:100); optimal dilutions for assays should be determined by the user.  | 
                                                
Purification:  | 
                                                    Protein A Purified  | 
                                                
Storage Buffer:  | 
                                                    PBS, 0.02% sodium azide  | 
                                                
Concentration:  | 
                                                    1 mg/ml  | 
                                                
Specificity:  | 
                                                    Detects the N-terminal domain of PUMA ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAβ.  | 
                                                
Storage:  | 
                                                    4ºC  | 
                                                
Shipping:  | 
                                                    Blue Ice or 4ºC  | 
                                                
Certificate of Analysis:  | 
                                                    2 µg/ml of SPC-165 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.  | 
                                                
Cellular Localization:  | 
                                                    Mitochondrion  | 
                                                
Tissue Specificity:  | 
                                                    N/A  | 
                                                
Accession Number:  | 
                                                    NP_001120712.1  | 
                                                
Gene ID:  | 
                                                    27113  | 
                                                
Swiss-Prot:  | 
                                                    Q9BXH1  | 
                                                
Field of Use:  | 
                                                    For in vitro research use only.  |