Puromycin Dihydrochloride | 1860

A nucleoside antibiotic. It inhibits protein synthesis by disrupting peptide transfer on ribosomes causing premature chain termination during translation. It is a potent translational inhibitor in both prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase gene (pac) from Streptomyces. Puromycin has a fast mode of action, causing rapid cell death at low antibiotic concentrations. Adherent mammalian cells are sensitive to concentrations of 2 to 5 µg/ml, while cells in suspension are sensitive to concentrations as low as 0.5 to 2 µg/ml.

Puromycin Dihydrochloride is a nucleoside antibiotic that inhibits protein synthesis by disrupting peptide transfer on ribosomes causing premature chain termination during translation. It is a potent translational inhibitor in both prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase gene (pac) from Streptomyces.

1860 | Puromycin Dihydrochloride DataSheet

Alternate Name/Synonyms: 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride

Appearance: White to off-white solid

Formulation: N/A

CAS Number: 58-58-2

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₂H₂₉N₇O₅ · 2HCl

Molecular Weight: 544.43

Cell-Permeable?: No

Purity: ≥98% by HPLC

Solubilities: H₂O (~50 mg/ml)

Handling: Protect from air and moisture

Country of Origin: USA

Tag Line: A protein synthesis inhibitor

MDL Number: MFCD00012691

PubChem CID: 16757772

SMILES: CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl

InChi: InChI=1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H

InChi Key: MKSVFGKWZLUTTO-UHFFFAOYSA-N