QTRT1 Antibody (C-term) [APR32921G]

QTRT1 Antibody (C-term) [APR32921G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This QTRT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 303-337 amino acids from the C-terminal region of human QTRT1.

Cellular Localisation: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03218}. Mitochondrion outer membrane {ECO:0000255|HAMAP-Rule:MF_03218}; Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_03218}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_03218}. Note=Weakly associates with mitochondria, possibly via QTRT2. {ECO:0000255|HAMAP- Rule:MF_03218}

Molecular Weight: 44048

Clone: Polyclonal

Gene Name: QTRT1 {ECO:0000255|HAMAP-Rule:MF_03218}

Gene ID: 81890

Function: Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU (N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7- ( ( (4, 5-cis-dihydroxy-2- cyclopenten-1-yl) amino) methyl) -7-deazaguanosine) (PubMed:11255023, PubMed:20354154) . Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity) .

Summary: N/A

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Queuine tRNA-ribosyltransferase, Guanine insertion enzyme, tRNA-guanine transglycosylase, QTRT1, TGT, TGUT