QuickDetect™ 5-lipoxygenase (5-LOX) (Human) ELISA Kit - E4704

E4704 | QuickDetect™ 5-lipoxygenase (5-LOX) (Human) ELISA Kit DataSheet

's 5-lipoxygenase ELISA kit is based on Sandwich ELISA method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 5-LOX. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 5-LOX is added to each Microelisa stripplate well and after incubation, all unbound components are washed away. The TMB substrate solution is added to each well. The wells that contain 5-LOX and HRP conjugated 5-LOX antibody will appear blue in color and turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 5-LOX. The concentration of 5-LOX in the samples can be calculated by comparing the OD of the samples to the standard curve.

Alternate Name : ALOX5, 5-LOX, Arachidonate 5-lipoxygenase

Tag Line : A Sandwich ELISA kit for the quantitative measurement of 5-lipoxygenase in human serum, plasma, culture media samples

Summary :

Detection Method : Absorbance-450 nm

Sample Type : Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids

Species Reactivity : Human

Applications : Detection range: 1U/L - 480 U/L, Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%, CV (%) = SD/mean X 100, Sensitivity: 1U/L

Features and Benefits : Detection of 5-lipoxygenase in human serum, plasma, culture media samples., Assay range 1U/L - 480 U/L, Sensitivityα 1U/L