QuickDetect™ Hydroxylysine (Human) ELISA Kit - E4714

E4714 | QuickDetect™ Hydroxylysine (Human) ELISA Kit DataSheet

Hydroxylysine (Hyl) is an amino acid which arises from a post-translational hydroxy modification of lysine. It is most widely known as a component of collagen. QuickDetect™ Hydroxylysine (Human) ELISA Kit uses a double-antibody sandwich enzyme-linked immunosorbent one-step process assay to assay the level of hydroxylysine (Hyl) in samples. Standard, test sample and HRP-labeled hydroxylysine (Hyl) antibodies were added to enzyme wells which are Pre-coated with hydroxylysine (Hyl) antibody, then carry out incubation and wash to remove the uncombined enzyme. Upon adding Chromogen Solution A and B, the color of the liquid will change into blue, and the reaction with the acid will cause the color to become yellow. The depth of color and the concentration of the hydroxylysine(Hyl) sample are positively correlated.

Alternate Name : hydroxylysine, Hyl

Tag Line : A rapid sandwich ELISA kit for the quantitative measurement of hydroxylysine in human serum, plasma, cell culture supernatant and other biological fluids

Summary :

Detection Method : Absorbance-450 nm

Sample Type : Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids, Tissue and cell lysates

Species Reactivity : Human

Applications : This ELISA kit is used for in vitro quantitative determination of Human Hydroxylysine, Assay range: 9.3ng/ml- 300ng/ml, Accuracy: Standard linear regression correlation coefficient R with the expected value of the concentration, greater than or equal to 0.9900.

Features and Benefits :