QuicKey Pro Human IGF-1 (Insulin-like Growth Factor 1) ELISA Kit | E-OSEL-H0012

QuicKey Pro Human IGF-1 (Insulin-like Growth Factor 1) ELISA Kit | E-OSEL-H0012 | Gentaur UK, US & Europe Distribution

Type: Sandwich

Assay Time: 1.5h

Sensitivity: 1.01ng/mL

Detection Range: 3.12-100ng/mL

UniProt ID: P05019

Target Name: IGF-1

Target Synonym: insulin-like growth factor 1, IGF1

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IGF1 are added to the micro ELISA plate wells. Human IGF1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IGF1. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IGF1. The concentration of Human IGF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Specifivity: This kit recognizes Human IGF1 in samples. No significant cross-reactivity or interference between Human IGF1 and analogues was observed.

Research Areas: Cancer, Developmental biology, Signal transduction

Storage: An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.