QuicKey Pro Mouse IgG (Immunoglobulin G) ELISA Kit | E-OSEL-M0005

QuicKey Pro Mouse IgG (Immunoglobulin G) ELISA Kit | E-OSEL-M0005 | Gentaur UK, US & Europe Distribution

Type: Sandwich

Assay Time: 1.5h

Sensitivity: 0.51ng/mL

Detection Range: 1.56-100ng/mL

UniProt ID: N/A

Target Name: IgG

Target Synonym: Immunoglobulin G, IgG

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG are added to the micro ELISA plate wells. Mouse IgG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse IgG. The concentration of Mouse IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Specifivity: This kit recognizes Mouse IgG in samples. No significant cross-reactivity or interference between Mouse IgG and analogues was observed.

Research Areas: Immunology

Storage: An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.