QuicKey Pro Porcine E3 (Estriol)ELISA Kit | E-OSEL-P0004

QuicKey Pro Porcine E3 (Estriol)ELISA Kit | E-OSEL-P0004 | Gentaur UK, US & Europe Distribution

Type: Competitive

Assay Time: 1.5h

Sensitivity: 1.12pg/mL

Detection Range: 3.13-200pg/mL

UniProt ID: N/A

Target Name: E3

Target Synonym: Estriol，E3

Test Principale: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Porcine E3 are added to the micro ELISA plate wells. Porcine E3 in samples (or standards) competes with a fixed amount of Porcine E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to Porcine E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Porcine E3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Specifivity: This kit recognizes Porcine E3 in samples. No significant cross-reactivity or interference between Porcine E3 and analogues was observed.

Research Areas: Cell Biology, Signal transduction, Developmental biology

Storage: An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.