RAB8A Antibody [AMM07495G]

RAB8A Antibody [AMM07495G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H, M

Specificity: This RAB8A monoclonal antibody is generated from mouse immunized with RAB8A recombinant protein.

Cellular Localisation: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Recycling endosome membrane. Cell projection, cilium. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:Q92930}; Lipid-anchor {ECO:0000250|UniProtKB:Q92930}; Cytoplasmic side {ECO:0000250|UniProtKB:Q92930}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:P55258}. Cytoplasm, cytoskeleton, cilium basal body. Midbody. Cytoplasm Cytoplasm, cytoskeleton, cilium axoneme Note=Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (PubMed:15837803) . In the GDP- bound form, present in the perinuclear region (PubMed:12221131) . Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form (PubMed:12221131) . Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis (PubMed:20890297) . Localizes to tubular recycling endosome (PubMed:19864458) . Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211) . Non-phosphorylated RAB8A predominantly localizes to the cytoplasm whereas phosphorylated RAB8A localizes to the membrane (PubMed:26824392, PubMed:29125462, PubMed:30398148) .

Molecular Weight: 23668

Clone: Monoclonal

Gene Name: RAB8A

Gene ID: 4218

Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297) . Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656) . May be involved in ciliogenesis (PubMed:21844891, PubMed:30398148) . Together with MICALL2, may also regulate adherens junction assembly (By similarity) . May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity) . Involved in autophagy (PubMed:27103069) .

Summary: N/A

Form: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000 WB--1:500~1:1000

Synonyms: Ras-related protein Rab-8A, Oncogene c-mel, RAB8A, MEL, RAB8