Rabbit AGC (Aggrecan) ELISA Kit | ELK6031

Rabbit AGC (Aggrecan) ELISA Kit | ELK6031 | Gentaur UK, US & Europe Distribution

Alternative Names: ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1

Species: Rabbit

Assay Type: Sandwich

Sensitivity: 0.239 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aggrecan (AGC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aggrecan (AGC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aggrecan (AGC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aggrecan (AGC) in the samples is then determined by comparing the OD of the samples to the standard curve.