Gentaur

Rabbit Anti-NoroVirus 1 Capsid Protein 1 | 15-MNV14-S

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SKU:
15-MNV14-S-GEN
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NULL490.00

Description

Rabbit Anti-NoroVirus 1 Capsid Protein 1 | 15-MNV14-S

Quality Level

300

biological source

goat

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

should not react with

human

technique(s)

direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)

shipped in

dry ice

General description

Goat anti-rabbit IgG-peroxidase conjugate associates with all rabbit Igs. The conjugate shows no reaction with human IgG by immunoelectrophoresis (IEP). Furthermore, the antibody is adsorbed with human IgG to reduce background staining with human samples.
 
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin.
 
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Specificity

Specificity of the Anti-Rabbit IgG-Peroxidase is determined by immunoelectrophoresis (IEP) using normal rabbit serum and rabbit IgG. The conjugate shows no reaction with human IgG by IEP.

Immunogen

Purified rabbit IgG

Application

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody has been used in western blotting , immunofluorescence staining , immunochemistry and immunoprecipitations.
 
Goat anti-rabbit IgG conjugated to peroxidase can be used for direct ELISA and immunohistochemistry.
Peroxidase-conjugated goat anti-rabbit IgG antibody was used for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution and yeast lysates following protein carbonylation assays at a dilution of 1:5000.
 
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat has been used as a secondary antibody during immunofluorescence staining.
 
Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.
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Additional Information

Size:
100 µL
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