Rabbit Cr (Calretinin) ELISA Kit | ELK9056

Rabbit Cr (Calretinin) ELISA Kit | ELK9056 | Gentaur UK, US & Europe Distribution

Alternative Names: CR; Calretinin; CALB2; CAB29; CAL2; 29 kDa calbindin; calbindin 2; calbindin 2; ; 29kD; calretinin; calbindin D29K

Species: Rabbit

Assay Type: Competitive Inhibition

Sensitivity: 0.75 nmol/ml

Standard: 80 nmol/ml

Detection Range: 1.25-80 nmol/ml

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 2h

Research Area: Tumor immunity;Neuro science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Calretinin (Cr) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calretinin (Cr) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calretinin (Cr) in the samples is then determined by comparing the OD of the samples to the standard curve.