Rabbit Cystatin C (Cys-C) ELISA Kit | ELK8290

Rabbit Cystatin C (Cys-C) ELISA Kit | ELK8290 | Gentaur UK, US & Europe Distribution

Alternative Names: Cys-C; Cystatin C; Gamma Trace; Post-Gamma-Globulin; Neuroendocrine Basic Polypeptide; Amyloid Angiopathy And Cerebral Hemorrhage

Species: Rabbit

Assay Type: Sandwich

Sensitivity: 0.3 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Kidney biomarker;Urology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cys-C (Cystatin C ) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cys-C (Cystatin C ) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cys-C (Cystatin C ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cys-C (Cystatin C ) in the samples is then determined by comparing the OD of the samples to the standard curve.