Description
Rabbit FDP (Fibrinogen Degradation Product) ELISA Kit | ELK2758 | Gentaur UK, US & Europe Distribution
Alternative Names: FnDP; Fibrin Split Products; Fibrin Degradation Product
Species: Rabbit
Assay Type: Competitive Inhibition
Sensitivity: 145.3 ng/mL
Standard: 30000 ng/mL
Detection Range: 468.75-30000 ng/mL
Sample Type: plasma
Assay Lenght: 2h
Research Area: Metabolic pathway;Hematology;Cardiovascular biology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Fibrinogen Degradation Product (FDP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fibrinogen Degradation Product (FDP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibrinogen Degradation Product (FDP) in the samples is then determined by comparing the OD of the samples to the standard curve.