Rabbit MMP2 (Matrix Metalloproteinase 2) ELISA Kit | ELK1950

Rabbit MMP2 (Matrix Metalloproteinase 2) ELISA Kit | ELK1950 | Gentaur UK, US & Europe Distribution

Alternative Names: CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase

Species: Rabbit

Assay Type: Sandwich

Sensitivity: 1.35 pg/mL

Standard: 200 pg/mL

Detection Range: 3.13-200 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 2 (MMP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Matrix Metalloproteinase 2 (MMP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 2 (MMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 2 (MMP2) in the samples is then determined by comparing the OD of the samples to the standard curve.