Description: This product is a rabbit monoclonal IgG and can be used as a matched isotype control for recombinant rabbit monoclonal antibodies. The RbNP15 IgG was selected as an isotype control following screening for low background on a variety of mouse and human cell types.

Applications Tested: This rabbit monoclonal isotype control has been tested for use as a negative control for flow cytometric analysis. Please use at the same concentration as the experimental antibody.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence.

Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts.

Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.