Rabbit SAA (Serum Amyloid A) ELISA Kit | ELK5375

Rabbit SAA (Serum Amyloid A) ELISA Kit | ELK5375 | Gentaur UK, US & Europe Distribution

Alternative Names: SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

Species: Rabbit

Assay Type: Competitive Inhibition

Sensitivity: 9 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Infection immunity;Kidney biomarker;Hepatology;Urology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Serum Amyloid A (SAA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Serum Amyloid A (SAA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serum Amyloid A (SAA) in the samples is then determined by comparing the OD of the samples to the standard curve.