RACGAP1 Antibody | 46-260

RACGAP1 Antibody | 46-260 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Dog, Cow

Immunogen: The immunogen for this antibody is: C-GRQGNFFASPMLK

Research Area: Cancer

Tested Application: E, WB, IHC, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0kDa according to NP_037409.2) . Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.Immunohistochemistry:In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to recognize all reported isoforms (NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1) . Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP_001119575.1, NP_001306932.1, NP_001306930.1, NP_001306933.1

Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: RACGAP1, MgcRacGAP, Rac GTPase activating protein 1, ID-GAP, MGCRACGAP, GTPase activating protein, HsCYK-4, HsCYK-4

User Note: N/A

BACKGROUND: N/A