Raf-B (phospho-S445) polyclonal Antibody | BS6338

Raf-B (phospho-S445) polyclonal Antibody | BS6338 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Rat

Application: WB

Application Range: WB 1:500 - 1:2000

Background: Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: P-Raf-B (S445) polyclonal Antibody detects endogenous levels of Raf-B protein when phosphorylated at Ser445.

Molecular Weight: ~ 110 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1; RAFB1

Immunogen: Synthetic phosphopeptide derived from human Raf-B around the phosphorylation site of Serine 445.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Signaling Pathways Activating p38 MAP Kinase,