Rat A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4) ELISA Kit | KTE100333

Rat A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4) ELISA Kit | KTE100333 | Gentaur UK, US & Europe Distribution

Application: This Rat A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS4 in samples. An antibody specific for ADAMTS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat A disintegrin and metalloproteinase with thrombospondin motifs 4 microplate
• Rat A disintegrin and metalloproteinase with thrombospondin motifs 4 standard
• Rat A disintegrin and metalloproteinase with thrombospondin motifs 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ADAMTS4. No significant cross-reactivity or interference between Rat ADAMTS4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: ADAMTS4 encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

Alternative Names: ADAMTS4; ADAMTS-2; ADAMTS-4; ADMP-1; KIAA0688; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 4; aggrecanase-1

Search name: ADAMTS4; ADAMTS-2; ADAMTS-4; ADMP-1; KIAA0688; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 4; aggrecanase-1

Tag: ADAMTS4