Rat AchE (Acetylcholinesterase) ELISA Kit | ELK5988

Rat AchE (Acetylcholinesterase) ELISA Kit | ELK5988 | Gentaur UK, US & Europe Distribution

Alternative Names: ARAChE; N-AChE; YT

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.34 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Metabolic pathway;Hematology;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetylcholinesterase (AchE) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Acetylcholinesterase (AchE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetylcholinesterase (AchE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acetylcholinesterase (AchE) in the samples is then determined by comparing the OD of the samples to the standard curve.