Rat Acly (ATP-citRate synthase) ELISA Kit | ELK8567

Rat Acly (ATP-citRate synthase) ELISA Kit | ELK8567 | Gentaur UK, US & Europe Distribution

Alternative Names: ACL; ACLY; ATP citrate (pro S ) lyase; ATP citrate lyase; ATP citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.266 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabonomics

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP-citRate synthase (Acly) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATP-citRate synthase (Acly) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP-citRate synthase (Acly), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP-citRate synthase (Acly) in the samples is then determined by comparing the OD of the samples to the standard curve.