Rat aCTx (Alpha-Crosslaps) ELISA Kit | ELK1542

Rat aCTx (Alpha-Crosslaps) ELISA Kit | ELK1542 | Gentaur UK, US & Europe Distribution

Alternative Names: Alpha CTx; a-CTXI; aCTX-I; A-Cr; Type I Collagen C-Telopeptide-Related Fraction

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 43.45 pg/mL

Standard: 9000 pg/mL

Detection Range: 140.63-9000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Bone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-Crosslaps (aCTx) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-Crosslaps (aCTx) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Crosslaps (aCTx) in the samples is then determined by comparing the OD of the samples to the standard curve.