Rat ADRbK2 (Adrenergic Receptor Beta Kinase 2) ELISA Kit | ELK7725

Rat ADRbK2 (Adrenergic Receptor Beta Kinase 2) ELISA Kit | ELK7725 | Gentaur UK, US & Europe Distribution

Alternative Names: B-ARK2; bARK2; Beta-ARK2; GRK3; G-protein-coupled receptor kinase 3

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.31 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenergic Receptor Beta Kinase 2 (ADRbK2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adrenergic Receptor Beta Kinase 2 (ADRbK2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenergic Receptor Beta Kinase 2 (ADRbK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenergic Receptor Beta Kinase 2 (ADRbK2) in the samples is then determined by comparing the OD of the samples to the standard curve.