Rat AFM (Afamin) ELISA Kit | ELK7030

Rat AFM (Afamin) ELISA Kit | ELK7030 | Gentaur UK, US & Europe Distribution

Alternative Names: ALB2; ALBA; ALF; A-ALB; Alpha-Albumin

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 0.93 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Tumor immunity;Infection immunity;Hepatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Afamin (AFM) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Afamin (AFM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Afamin (AFM) in the samples is then determined by comparing the OD of the samples to the standard curve.