Rat AGT (Angiotensinogen) ELISA Kit | ELK4678

Rat AGT (Angiotensinogen) ELISA Kit | ELK4678 | Gentaur UK, US & Europe Distribution

Alternative Names: ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor, Clade A, Member 8 (Alpha-1 Antiproteinase, Antitrypsin)

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.35 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensinogen (AGT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Angiotensinogen (AGT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensinogen (AGT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensinogen (AGT) in the samples is then determined by comparing the OD of the samples to the standard curve.