Rat ALPL (Alkaline Phosphatase, Liver/Bone/Kidney) ELISA Kit | ELK5682

Rat ALPL (Alkaline Phosphatase, Liver/Bone/Kidney) ELISA Kit | ELK5682 | Gentaur UK, US & Europe Distribution

Alternative Names: BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase, Tissue-Nonspecific Isozyme

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.3 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Hepatology;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Liver/Bone/Kidney (ALPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) in the samples is then determined by comparing the OD of the samples to the standard curve.