Rat Anti-glucoprotein 210 antibody (GP210-Ab) ELISA Kit | KTE100930

Rat Anti-glucoprotein 210 antibody (GP210-Ab) ELISA Kit | KTE100930 | Gentaur UK, US & Europe Distribution

Application: This Rat Anti-glucoprotein 210 antibody (GP210-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GP210-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated GP210-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled GP210-Ab and unlabeled GP210-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GP210-Ab in the sample. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Anti-glucoprotein 210 antibody microplate
• Rat Anti-glucoprotein 210 antibody standard
• Rat Anti-glucoprotein 210 antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Anti-glucoprotein 210 antibody (GP210-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Rat GP210-Ab. No significant cross-reactivity or interference between Rat GP210-Ab and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Nuclear pore glycoprotein-210 (gp210) is an essential trafficking regulator in the eukaryotic nuclear pore complex. Gp-210 anchors the pore complex to the nuclear membrane. and protein tagging reveals its primarily located on the luminal side of double layer membrane at the pore. A single polypeptide motif of gp210 is responsible for sorting to nuclear membrane, and indicate the carboxyl tail of the protein is oriented toward the cytoplasmic side of the membrane.The deduced protein contains 1, 886 amino acids. Northern blot analysis detected a 7.5-kb transcript. In situ hybridization detected preferential expression in epithelial cells. In kidney, expression was seen in both the epithelium derived from the ureteric tree and the mesenchyme-derived epithelium.

Alternative Names: GP210-Ab

Search name: GP210-Ab

Tag: GP210