Rat Anti-Mouse Sonic Hedgehog Antibody | 103-M476

Rat Anti-Mouse Sonic Hedgehog Antibody | 103-M476 | Gentaur UK, US & Europe Distribution

Species: Anti-Mouse

Host / biotech: Rat

Comment: N/A

Label: N/A

Clone / Antibody feature: (#6K12)

Subcategory: Monoclonal Antibody

Category: Antibody

Synonyms: Shh; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik

Isotype: IgG2

Application: WB, IHC (F), N

Detection Range: N/A

Species Reactivity/Cross reactivity: Mouse

Antigen: recombinant mouse Shh (N-terminal) fragment

Description: Human Shh cDNA encodes a 462 amino acid (aa) residue (45 kDa) precursor protein with a 23 aa signal peptide. An autocatalytic cleavage reaction yields a 19 kDa (residues 24 - 197) amino-terminal fragment (Shh-N), and a 25 kDa (residues 198 - 462) carboxy-terminal domain (Shh-C). The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N. When Shh is expressed in insect or mammalian cells, a palmitoyl group is also attached to the N-terminal cysteine of Shh-N via an amide linkage. Although the binding affinity to their receptors is not changed, lipid-modified Shh-N proteins are more potent than the unmodified proteins in cell-based assays. Other hydrophobic modifications to unmodified Shh-N, including the substitution of the N-terminal cysteine residue with two hydrophobic isoleucine residues, can also increase Shh-N potency.

Purity Confirmation: N/A

Endotoxin: N/A

Formulation: lyophilized

Storage Handling Stability: Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

Reconstituation: Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Molecular Weight: N/A

Lenght (aa): N/A

Protein Sequence: N/A

NCBI Gene ID: 20423